Single Radial Hemolysis Technique in Comparison with Indirect Hemagglutination and Agar Gel Precipitation tests for Assaying Antibodies against Infectious Bursal Disease Virus

نویسندگان

  • M. A. Zahoor
  • M. S. Mehmood
  • M. Akhtar
چکیده

The interaction between antibodies and the surface antigens of infectious bursal disease virus particles was studied in the agarose gel by using single radial hemolysis (SRH) test. Sonicated antigen was used to sensitize the Human "O" erythrocytes. Hemolytic zones, using the filter paper discs were observed after overnight incubation in humid chamber at 37C. Indirect Hemagglutination (IHA) and agar gel precipitation tests (AGPT) were also performed and compared with SRH. Out of 50 serum samples, the percentage of positive samples using SRH, IHA and AGP tests were 70, 58 and 38 respectively, which showed the sensitivity in decreasing order. Results revealed that SRH is simple, sensitive, quick and particularly suitable for the routine screening of serum samples and assessing the immune status of birds against infectious bursal disease virus.

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تاریخ انتشار 2014